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JoVE Journal Bioengineering
Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota
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Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota

Article DOI: 10.3791/59054-v • 06:23 min • February 15th, 2019
February 15th, 2019 •
1, 1, 2, 3, 3, 1
1Dairy and Functional Foods Research Unit, Eastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, 2ProDigest, 3Division of Gastroenterology, Hepatology, and Nutrition, Children's Hospital of Philadelphia

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Summary
Automatic Translation

Here, we present a protocol for culturing the gut microbiota of the colon in vitro, using a series of bioreactors that simulate the physiological conditions of the gastro intestinal tract.

Tags

Advanced In Vitro Culturing Technology Gut Microbiota Microbiota Dynamics Multi-stage In Vitro System Colon Regions Defined Medium Mucin Agricarriers PH Adjustment Nitrogen Flush Fecal Homogenate Overnight Culture Sample Port Bioreactor
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