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JoVE Journal Bioengineering
Construction and Use of an Electrical Stimulation Chamber for Enhancing Osteogenic Differentiation in Mesenchymal Stem/Stromal Cells In Vitro
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Construction and Use of an Electrical Stimulation Chamber for Enhancing Osteogenic Differentiation in Mesenchymal Stem/Stromal Cells In Vitro

Article DOI: 10.3791/59127-v • 08:11 min • January 31st, 2019
January 31st, 2019 •
1, 1, 1, 1, 1,2, 1
1Frankfurt Initiative for Regenerative Medicine, Johann Wolfgang Goethe-University, 2J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida

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Summary
Automatic Translation

Here we present a protocol for the construction of a cell culture chamber designed to expose cells to various types of electrical stimulation, and its use in treating mesenchymal stem cells to enhance osteogenic differentiation.

Tags

Electrical Stimulation Chamber Osteogenic Differentiation Mesenchymal Stem Cells Bone Tissue Engineering Cell Types Electro-sensitive Cell Behaviors Platinum Wire Silver-coated Wire Six Well Plate Lid Super-conductive Glue
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