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Detached Leaf Assays to Simplify Gene Expression Studies in Potato During Infestation by Chewing Insect Manduca sexta
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Detached Leaf Assays to Simplify Gene Expression Studies in Potato During Infestation by Chewing Insect Manduca sexta

Article DOI: 10.3791/59153-v • 05:56 min • May 15th, 2019
May 15th, 2019 •
1, 2, 2, 1
1Invasive Insect Biocontrol and Behavior Lab, United States Department of Agriculture (USDA), Agricultural Research Service (ARS), 2Genetic Improvement for Fruits and Vegetables Lab, USDA-ARS

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Summary
Automatic Translation

The presented method creates natural herbivore damaged plant tissue through the application of Manduca sexta larvae to detached leaves of potato. The plant tissue is assayed for expression of six transcription factor homologs involved in early responses to insect herbivory.

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Detached Leaf Assays Gene Expression Studies Potato Chewing Insect Manduca Sexta Streamlined Approach Plant Gene Expression Infestation Reproducible Data Plant/insect Interactions Insect Feeding Larvae Supply Healthy Plants Visualizing Healthy Plants And Insects Molecular Biologist Genetic Improvement Of Fruits And Vegetables Lab Nodal Propagated Tissue Culture Potato Plants
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