JoVE Journal
Genetics
Genetics
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
Here, we present an enhanced yeast one-hybrid screening protocol to identify the transcription factors (TFs) that can bind to a human DNA region of interest. This method uses a high-throughput screening pipeline that can interrogate the binding of >1,000 TFs in a single experiment.