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Journal / Bioengineering / Growth and Characterization of Irradiated…
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Bioengineering

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JoVE Journal Bioengineering
Growth and Characterization of Irradiated Organoids from Mammary Glands
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Growth and Characterization of Irradiated Organoids from Mammary Glands

Article DOI: 10.3791/59293-v • 09:49 min • May 3rd, 2019
May 3rd, 2019 •
1, 1, 1, 1,2,3
1Department of Chemical and Biomolecular Engineering, Vanderbilt University, 2Department of Biomedical Engineering, Vanderbilt University, 3Department of Radiation Oncology, Vanderbilt University Medical Center

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Summary
Automatic Translation

Organoids developed from mouse mammary glands were irradiated and characterized to assess epithelial traits and interactions with immune cells. Irradiated organoids can be used to better evaluate cell-cell interactions that may lead to tumor cell recruitment in irradiated normal tissue.

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Growth Characterization Irradiated Organoids Mammary Glands Ionizing Radiation Tumor Cell Recruitment Cancer Recurrence Mammary Organoids In Vivo Characteristics Biological Variables Low-adhesion Plates Protein Matrices Triple-negative Breast Cancer Local Regional Recurrence Radiation Influence Tumor Behavior Immune Cell Behavior Confocal Microscopy Removal Of Mammary Glands Irradiation Of Samples Sterile Cell Plate Mince With Scalpels Collagenase Solution Water Bath
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