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Journal / Biochemistry / Robust Comparison of Protein Levels Across…
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JoVE Journal Biochemistry
Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting
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Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting

Article DOI: 10.3791/59438-v • 08:13 min • April 9th, 2019
April 9th, 2019 •
1,2, *1,2, *1,2, *1,2, 1, 1,2, 1,2
1Centre for Discovery Brain Sciences, University of Edinburgh, 2Euan MacDonald Centre for Motor Neurone Disease Research, University of Edinburgh
* These authors contributed equally

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Summary
Automatic Translation

This method describes a robust and reproducible approach for the comparison of protein levels in different tissues and at different developmental timepoints using a standardized quantitative western blotting approach.

Tags

Quantitative Western Blotting Protein Levels Tissues Development Fluorescent Total Protein Stain Internal Loading Control Snap-frozen Samples Protein Extraction Centrifugation Protein Concentration Bovine Serum Albumin Standards 96-well Optical Plate Absorption Measurement
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