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Assessment of Zebrafish Lens Nucleus Localization and Sutural Integrity
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Assessment of Zebrafish Lens Nucleus Localization and Sutural Integrity

Article DOI: 10.3791/59528-v • 07:16 min • May 6th, 2019
May 6th, 2019 •
1,2, 1, 2
1Department of Physiology and Biophysics, University of California, Irvine, 2Department of Developmental and Cell Biology, University of California, Irvine

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These protocols were developed to analyze cortical lens morphology, structural integrity of the zebrafish lens sutures in fixed and live lenses and to measure the position of the zebrafish lens nucleus along the anterior-posterior axis.

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Zebrafish Lens Nucleus Localization Sutural Integrity Functional Studies Lens Development Lens Physiology Reverse Genetic Screening Lens Phenotypes Zebrafish Lens Membrane Morphology Quantifying Lens Development Zebrafish Lens Optics Mechanisms Controlling Lens Development Normal Physiology Pathophysiology Cataract Prevention Asymmetrically Localized Lens Nuclei Identifying Lens Sutures Sedation With Tricaine Microdissection Scissors Excising Zebrafish Eyes Custom-made Petri Dish Silicone Dissection Mold PBS Solution Harvesting Zebrafish Lens
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