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JoVE Journal Developmental Biology
Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells
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Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells

Article DOI: 10.3791/59561-v • 09:29 min • July 10th, 2019
July 10th, 2019 •
1,2, 1,2,3, 1,2,3
1Center for Neuroscience and Regenerative Medicine, Uniformed Services University, 2Department of Anatomy, Physiology, and Genetics, Uniformed Services University, 3Graduate Program in Neuroscience, Uniformed Services University

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Summary
Automatic Translation

This protocol demonstrates methodologies for a mouse model of open-skull traumatic brain injury and transplantation of cultured human induced pluripotent stem cell-derived cells into the injury site. Behavioral and histologic tests of outcomes from these procedures are also described in brief.

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Controlled Cortical Impact Model Mouse Brain Injury Therapeutic Transplantation Human Induced Pluripotent Stem Cells Neural Cells Preclinical Workflow Traumatic Brain Injury Mechanical Force Injury Parameters Well-defined Injury Cranial Drilling Craniectomy Stereotaxic Frame Antibiotic Ophthalmic Ointment Iodine-based Solution Midline Incision Sterile Cotton Swabs Fascia Impactor Probe
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