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An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
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An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Article DOI: 10.3791/59628-v • 09:45 min • May 21st, 2019
May 21st, 2019 •
1, 1, 1, 1
1Department of Biomedical Science and Research Institute for Bioscience & Biotechnology, Hallym University

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This protocol uses a probe-based real-time polymerase chain reaction (PCR), a sulforhodamine B (SRB) assay, 3’ untranslated regions (3’ UTR) cloning, and a luciferase assay to verify the target genes of a miRNA of interest and to understand the functions of miRNAs.

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In Vitro Protocol Evaluating MicroRNA Levels Functions Target Genes Tumor Cells Neural MicroRNA Target Interactions MicroRNA Functions MicroRNA Regulation Biological Processes Healthy States Diseased States MicroRNA Targets Functional MicroRNAs Validation Of Direct Target Cancer Therapy Half-maximal Inhibitory Concentration Anti-cancer Drugs Evaluation Cell Counter 96 Well Plate Transfection Mixtures MicroRNA Control Mimic MicroRNA-107 Mimic Serum Media Transfection Reagent
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