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JoVE Journal Developmental Biology
Fast and Efficient Expression of Multiple Proteins in Avian Embryos Using mRNA Electroporation
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Fast and Efficient Expression of Multiple Proteins in Avian Embryos Using mRNA Electroporation

Article DOI: 10.3791/59664-v • 09:40 min • June 7th, 2019
June 7th, 2019 •
1, 2,3, 1,2,3
1Department of Biological Sciences, University of Southern California, Los Angeles, 2Department of Radiology and Developmental Neuroscience Program, Saban Research Institute, Children's Hospital Los Angeles, 3Keck School of Medicine, Department of Radiology, University of Southern California, Los Angeles

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We report messenger RNA (mRNA) electroporation as a method that permits fast and efficient expression of multiple proteins in the quail embryo model system. This method can be used to fluorescently label cells and record their in vivo movements by time-lapse microscopy shortly after electroporation.

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MRNA Electroporation Avian Embryos Protein Expression Fluorescent Proteins DNA Electroporation Genetic Payloads Plasma Membrane Transfer Of RNA And DNA Embryonic Developmental Stage Quail Egg Petri Dish Albumen Filter Paper Pasteur Pipette PPS
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