Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Identification of Circular RNAs using RNA…
JoVE Journal
Genetics

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Genetics
Identification of Circular RNAs using RNA Sequencing
A Universal Protocol for Large-scale gRNA Library Production from any DNA Source
Journal (Genetics)
A Universal Protocol for Large-scale gRNA Library Production from any DNA Source
Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen
Journal (Genetics)
Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen
 

Identification of Circular RNAs using RNA Sequencing

Article DOI: 10.3791/59981-v • 08:25 min • November 14th, 2019
November 14th, 2019 •
1,2, 1,2, 1,2, 1,2, 2,3, 2,3, 1,2
1Translational Genomic Research Institute, 2Arizona Alzheimer's Consortium, 3Banner Sun Health Research Institute

Chapters

Summary
Automatic Translation

Circular RNAs (circRNAs) are non-coding RNAs that may have roles in transcriptional regulation and mediating interactions between proteins. Following assessment of different parameters for construction of circRNA sequencing libraries, a protocol was compiled utilizing stranded total RNA library preparation with RNase R pre-treatment and is presented here.

Tags

Identification Circular RNAs RNA Sequencing CircRNA Library Preparation Kit Type RNase R Treatment Input Amounts CircRNA Detection Adjustable Parameters Sample Types Expression Distribution Functional Role Total RNA Sample RIN Value DV200 Value Agilent Bioanalyzer TapeStation Diluting Total RNA RNase-free Water RNase R Buffer Working Concentration Reaction Tube Diluted RNase R Pipetting Up And Down 37-degree Celsius Water Bath
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter