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Optische clearing en beeldvorming van Immunolabeled nierweefsel
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Optische clearing en beeldvorming van Immunolabeled nierweefsel

Article DOI: 10.3791/60002-v • 06:18 min • July 22nd, 2019
July 22nd, 2019 •
1, 1,2,3, 4, 4,5,6, 1,7
1Division of Nephrology and Clinical Immunology, University Hospital RWTH Aachen, 2III. Department of Medicine, University Medical Center, Hamburg-Eppendorf, 3Department of Nephrology, Monash Health, 4Division of Nephrology and Hypertension, Oregon Health and Science University, 5Renal Section, Veterans Affairs Portland Health Care System, 6Fondation LeDucq Transatlantic Networks of Excellence, 7Department of Internal Medicine, Nephrology and Transplantation, Erasmus Medical Center

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De combinatie van antilichaam etikettering, optische clearing en geavanceerde Lichtmicroscopie maakt een driedimensionale analyse van complete structuren of organen mogelijk. Hier beschreven is een eenvoudige methode om immunolabeling van dikke nierschijfjes te combineren, optische clearing met ethyl CINNAMAAT, en confocale beeldvorming die visualisatie en kwantificering van driedimensionale nierstructuren mogelijk maakt.

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Geneeskunde uitgave 149 optische clearing whole-Mount immunolabeling nier tubulus weefsel clearing driedimensionale analyse
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