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Isolation of Intact Eyeball to Obtain Integral Ocular Surface Tissue for Histological Examination and Immunohistochemistry
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Isolation of Intact Eyeball to Obtain Integral Ocular Surface Tissue for Histological Examination and Immunohistochemistry

Article DOI: 10.3791/60086-v • 05:15 min • October 20th, 2019
October 20th, 2019 •
1, 1
1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University

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Summary
Automatic Translation

This protocol describes a method for the isolation of the mouse eyeball with eyelid, ocular surface, anterior and posterior segments in relatively intact position.

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Intact Eyeball Ocular Surface Tissue Histological Examination Immunohistochemistry Protocol Skull Removal Orbital Bones Removal Eyelids Lens Retina Ophthalmologists Dianlel Guo Dissection Technique Ocular Surface Epithelial Sheet Histology Immunostaining Soft Tissues Scar Marks Euthanizing The Mouse Straight Scissors Sagittal Midline Intraparietal Bone Nasal Petri Dish Jaw Removal Tongue Removal Optic Nerve Removal Brain Tissues Removal
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