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Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Article DOI: 10.3791/60503-v • 09:20 min • December 18th, 2019
December 18th, 2019 •
1, 1, 1,2, 1, 1
1Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, 2Icahn Institute for Data Science and Genomic Technology, Icahn School of Medicine at Mount Sinai

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This protocol describes laser capture microdissection for the isolation of cartilage and bone from fresh frozen sections of the mouse embryo. Cartilage and bone can be rapidly visualized by cresyl violet staining and collected precisely to yield high quality RNA for transcriptomic analysis.

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Laser Capture Microdissection Mouse Embryonic Cartilage Bone Gene Expression Analysis Heterogeneity Of Skeletal Structures Precise Isolation Adjacent Tissues RNA Integrity Cresyl Violet Staining Visualization Tissue Collection RNA Quality Cryosectioning Embedding Mold Cutting Temperature Compound Rapid Freezing Dry Ice Methyl-2-butene Bath Lidded Container Cryostat Specimen Holder
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