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Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

Article DOI: 10.3791/60697-v • 10:19 min • February 3rd, 2020
February 3rd, 2020 •
1, 1, 1, 1, 1,2, 3, 1
1Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, 2College of Agriculture, Yangtze University, 3Laboratory of Molecular Genetics, China National Tobacco Corporation, Guizhou Institute of Tobacco Science

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Summary
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Here, we present a modified screening method that can be extensively used to quickly screen RNA silencing suppressors in plant pathogens.

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Screening Identification RNA Silencing Suppressors Secreted Effectors Plant Pathogens Technique Rapid Accurate Extensive Screening Assay Potting Soil Mixes Peat Moss Perlite Vermiculite Autoclave Plant Fertilizer Solution Nicotiana Bethamiana 16c Seeds Plastic Dome Germinate Light And Temperature-controlled Growth Chambers Relative Humidity Photoperiod Seedlings Water Fertilizer Growth
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