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JoVE Journal Biology
Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ
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Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ

Article DOI: 10.3791/60859-v • 10:05 min • May 8th, 2020
May 8th, 2020 •
1,2, 3, 1,4, 1, 5
1Ramón y Cajal Institute for Health Research (IRYCIS), Ramón y Cajal University Hospital, 2Centro Integrativo de Biología y Química Aplicada (CIBQA), Universidad Bernardo O'Higgins, 3Department of Molecular Biology, Faculty of Sciences, Universidad Autónoma de Madrid (UAM), 4Instituto de Investigaciones Biosanitarias, Facultad de Ciencias Experimentales, Universidad Francisco de Vitoria, 5Department of Radiology, Rehabilitation & Physiotherapy, Faculty of Medicine, Complutense University

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Summary
Automatic Translation

The aim of this protocol is to induce transient in vivo production of nonlethal levels of reactive oxygen species (ROS) in mouse skin, further promoting physiological responses in the tissue.

Tags

Biology Protoporphyrin IX-dependent Photogeneration Reactive Oxygen Species (ROS) In Situ Methodology Experimental Tool Transient Activation Endogenous ROS Production Red Light Source Physiological Stimulating ROS Levels Skin Regeneration Therapy Healing Hair Growth ROS Biology And Signaling Research Hotpot Controlled Endogenous ROS Production User-friendly Calibration Chemical Precursors Protocol
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