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Slicing and Culturing Pig Hearts under Physiological Conditions

Article DOI: 10.3791/60913-v • 07:54 min • March 20th, 2020
March 20th, 2020 •
*1, *1, 1, 1, 2, 2, 2, 2, 1, 1,3,4,5,6
1Institute of Molecular Cardiology, Department of Medicine, University of Louisville, 2Department of Bioengineering, University of Louisville, 3Diabetes and Obesity Center, Department of Medicine, University of Louisville, 4Department of Pharmacology and Toxicology, University of Louisville, 5Institute of Cardiovascular Sciences, University of Manchester, 6Faculty of Pharmacy, Zagazig University
* These authors contributed equally

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Summary
Automatic Translation

This protocol describes how to slice and culture heart tissue under physiological conditions for 6 days. This culture system could be used as a platform for testing the efficacy of novel heart failure therapeutics as well as reliable testing of acute cardiotoxicity in a 3D heart model.

Tags

Slicing Culturing Pig Hearts Physiological Conditions Cardiac Toxicity Testing Heart Failure Therapies Culture System Predictive Model Pre-clinical Testing Clinical Testing Lab Manager Vibratome Tyrode Solution Cardioplegic Solution Dissect Left Ventricle Tissue Block Razor Blades Agar Block Tissue Glue
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