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JoVE Journal Neuroscience
Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System
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Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System

Article DOI: 10.3791/60993-v • 10:12 min • May 5th, 2020
May 5th, 2020 •
*1, *1, *1, 1, 1,2
1Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, 2Sagol School of Neuroscience, Tel Aviv University
* These authors contributed equally

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Summary
Automatic Translation

Axonal transport is a crucial mechanism for motor neuron health. In this protocol we provide a detailed method for tracking the axonal transport of acidic compartments and mitochondria in motor neuron axons using microfluidic chambers.

Tags

Axonal Transport Organelles Motor Neuron Cultures Microfluidic Chambers System Neuronal Health Neuronal Disease Models Spatial Temporal Control Axonal Biology Neurodegenerative Disease ALS Basic Mechanisms Therapies Repair Axonal Transport Defects Axonal Research Axonal Growth Axonal Degeneration PDMS Casting Wafer Platform Liquid Nitrogen Syringe
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