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Journal / Genetics / Vi introduserer punktmutasjoner i menneskelige…
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Genetics

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JoVE Journal Genetics
Vi introduserer punktmutasjoner i menneskelige pluripotente stamceller ved hjelp av sømløs genomredigering
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Vi introduserer punktmutasjoner i menneskelige pluripotente stamceller ved hjelp av sømløs genomredigering

Article DOI: 10.3791/61152-v • 09:03 min • May 10th, 2020
May 10th, 2020 •
1, 1, 1
1MRC Centre for Regenerative Medicine, University of Edinburgh

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Her beskriver vi en detaljert metode for sømløs genredigering i humane pluripotente stamceller ved hjelp av et piggyBac-basert donorplasmid og Cas9 nickasemutanten. To punktmutasjoner ble introdusert i ekson 8 av hepatocyttkjernefaktoren 4 alfa (HNF4α) locus i humane embryonale stamceller (hESCs).

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Genetikk genomredigering CRISPR sømløse pluripotente stamceller piggyBac knock-in
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