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In Vivo Quantification of Protein Turnover in Aging C. Elegans using Photoconvertible Dendra2

Article DOI: 10.3791/61196-v • 09:45 min • June 13th, 2020
June 13th, 2020 •
1,2, 1,3
1Leibniz Research Institute for Molecular Pharmacology im Forschungsverbund, 2NeuroCure Cluster of Excellence, Charité - Universitätsmedizin Berlin, 3Department of Biology and Chemistry, University of Bremen

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Summary
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Presented here is a protocol to monitor degradation of the protein huntingtin fused to the photoconvertible fluorophore Dendra2.

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In Vivo Protein Turnover Aging C. Elegans Photoconvertible Dendra2 Tracking Quantification Degradation Noninvasive Technique Microscopy Set Up Fluorophore Adaptable To Mammalian Systems Zebrafish Proteostasis Network Transport And Trafficking Blue Light Laser Acquisition Settings Fusion Protein Nematodes Imaging Experiment Agarose Glass Slide Confocal Microscope Software Excitation And Emission
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