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Anvendelse af Laser Microdissection at afdække regionale Transcriptomics i Human Nyre Tissue
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Anvendelse af Laser Microdissection at afdække regionale Transcriptomics i Human Nyre Tissue

Article DOI: 10.3791/61371-v • 05:46 min • June 9th, 2020
June 9th, 2020 •
1, 1, 1, 1,2, 1, 1, 1, 3, 3, 4, 1, 1, 1, For The Kidney Precision Medicine Project,
1Department of Medicine, Indiana University School of Medicine, 2Department of Cellular & Integrative Physiology, Indiana University School of Medicine, 3Division of Nephrology, Department of Medicine, Ohio State University Wexner Medical Center, 4Division of Pathology, Indiana University School of Medicine

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Vi beskriver en protokol for laser mikrodissementering af undersegmenter af den menneskelige nyre, herunder glomerulus, proksimal tubule, tykt stigende lem, opsamlingskanal og interstitium. RNA'et isoleres derefter fra de opnåede rum, og der udføres RNA-sekventering for at bestemme ændringer i transskriptionssignaturen inden for hvert undersegment.

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Biologi Problem 160 Laser Microdissection LMD protokol nyre glomerulus rørformet undersegment
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