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Journal / Immunology and Infection / Fäkale (Mikro-) RNA-Isolierung
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Immunology and Infection

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Fäkale (Mikro-) RNA-Isolierung
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Fäkale (Mikro-) RNA-Isolierung

Article DOI: 10.3791/61908-v • 05:35 min • October 28th, 2020
October 28th, 2020 •
1,2, 1,2, 1,2
1Ann Romney Center for Neurologic Diseases, Department of Neurology, Partners Multiple Sclerosis Center, Brigham and Women's Hospital and Harvard Medical School, 2Evergrande Center for Immunologic Diseases, Harvard Medical School and Brigham and Women's Hospital

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Dieses Protokoll isoliert hochwertige Gesamt-RNA aus Kotproben von Tieren und Menschen. Ein kommerzielles miRNA-Isolierkit wird mit signifikanter Adaption verwendet, um reine RNA mit optimierter Quantität und Qualität zu isolieren. Die RNA-Isolate eignen sich für die meisten nachgeschalteten RNA-Assays wie Sequenzierung, Micro-Array und RT-PCR.

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Immunologie und Infektion Ausgabe 164 microRNA Stuhlproben Stuhlproben RNA-Isolierung Gesamt-RNA miRNA
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