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Journal / Immunology and Infection / 분변(마이크로) RNA 분리
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Immunology and Infection

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JoVE Journal Immunology and Infection
분변(마이크로) RNA 분리
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분변(마이크로) RNA 분리

Article DOI: 10.3791/61908-v • 05:35 min • October 28th, 2020
October 28th, 2020 •
1,2, 1,2, 1,2
1Ann Romney Center for Neurologic Diseases, Department of Neurology, Partners Multiple Sclerosis Center, Brigham and Women's Hospital and Harvard Medical School, 2Evergrande Center for Immunologic Diseases, Harvard Medical School and Brigham and Women's Hospital

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이 프로토콜은 동물 및 인간 피험자의 분변 샘플에서 고품질 총 RNA를 분리합니다. 상용 miRNA 분리 키트는 최적화된 양과 품질로 순수 RNA를 분리하기 위해 상당한 적응과 함께 사용됩니다. RNA 분리는 시퀀싱, 마이크로 어레이 및 RT-PCR과 같은 대부분의 다운스트림 RNA 분석에 적합합니다.

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면역학 및 감염 문제 164 마이크로 RNA 대변 샘플 대변 표본 RNA 분리 총 RNA miRNA
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