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Journal / Immunology and Infection / Isolamento de RNA fecal (micro)
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JoVE Journal Immunology and Infection
Isolamento de RNA fecal (micro)
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Isolamento de RNA fecal (micro)

Article DOI: 10.3791/61908-v • 05:35 min • October 28th, 2020
October 28th, 2020 •
1,2, 1,2, 1,2
1Ann Romney Center for Neurologic Diseases, Department of Neurology, Partners Multiple Sclerosis Center, Brigham and Women's Hospital and Harvard Medical School, 2Evergrande Center for Immunologic Diseases, Harvard Medical School and Brigham and Women's Hospital

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Este protocolo isola RNA total de alta qualidade de amostras fecais de animais e seres humanos. Um kit comercial de isolamento de miRNA é usado com adaptação significativa para isolar o RNA puro com quantidade e qualidade otimizadas. Os isolados de RNA são bons para a maioria dos ensaios de RNA a jusante, como sequenciamento, micro-array e RT-PCR.

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Imunologia e Infecção Edição 164 microRNA amostras fecais amostras fecais isolamento de RNA RNA total miRNA
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