Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Xenopus laevis Egg Extract Preparation and Live…
JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology
Xenopus laevis Egg Extract Preparation and Live Imaging Methods for Visualizing Dynamic Cytoplasmic Organization
Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs
Journal (Biology)
Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs
The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry
Journal (Biology)
The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry
 

Xenopus laevis Egg Extract Preparation and Live Imaging Methods for Visualizing Dynamic Cytoplasmic Organization

Article DOI: 10.3791/61923-v • 05:58 min • June 6th, 2021
June 6th, 2021 •
1,2, 1,3
1Department of Chemical and Systems Biology, Stanford University School of Medicine, 2Current Address: Department of Biological Sciences, University of Southern California, 3Department of Biochemistry, Stanford University School of Medicine

Chapters

Summary
Automatic Translation

We describe a method for the preparation and live imaging of undiluted cytoplasmic extracts from Xenopus laevis eggs.

Tags

Xenopus Laevis Egg Extract Preparation Live Imaging Methods Cytoplasmic Dynamics Spatial Pattern Information Cost Effective Easy To Implement FEP Adhesive Tape Glass Slide Roller Applicator FEP Tape Coated Cover Slips Double-sided Sticky Imaging Spacer Glass Beaker Egg-laying Buffer Dejellying Solution Tightly Packed Eggs 0.25x MMR Buffer
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter