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Estimation of Crystalline Cellulose Content of Plant Biomass using the Updegraff Method
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Estimation of Crystalline Cellulose Content of Plant Biomass using the Updegraff Method

Article DOI: 10.3791/62031-v • 12:34 min • May 15th, 2021
May 15th, 2021 •
1, 2, 2
1Department of Plant and Soil Science, Texas Tech University, 2Fiber and Biopolymer Research Institute (FBRI), Department of Plant and Soil Science, Texas Tech University

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The Updegraff method is the most widely used method for the cellulose estimation. The main purpose of this demonstration is to provide a detailed Updegraff protocol for estimation of cellulose content in plant biomass samples.

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Updegraff Method Crystalline Cellulose Content Plant Biomass Estimation Glucose Monomers Beta-1 4 Glycosidic Bonds Protocol Phases Plant Material Crude Cell Wall Extraction Updegraff Treatment Acetic Acid Nitric Acid Hemicellulose Removal Lignin Removal Acid Hydrolysis Sulfuric Acid Glucose Monomers Production Anthrone Assay Greenhouse Washing Air Drying Tissue Separation Incubation Cutting Into Pieces Grinding Powder
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