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Developmental Biology

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JoVE Journal Developmental Biology
Generation and Culture of Lingual Organoids Derived from Adult Mouse Taste Stem Cells
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Generation and Culture of Lingual Organoids Derived from Adult Mouse Taste Stem Cells

Article DOI: 10.3791/62300-v • 07:57 min • April 5th, 2021
April 5th, 2021 •
*1, *1, 1, 1, 1, 1
1Department of Cell and Developmental Biology and the Rocky Mountain Taste and Smell Center, University of Colorado Anschutz Medical Campus
* These authors contributed equally

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Summary
Automatic Translation

The protocol presents a method for culturing and processing lingual organoids derived from taste stem cells isolated from the posterior taste papilla of adult mice.

Tags

Organoids Taste Stem Cells Tongue Model Drug Screening Biological Processes In Vitro Technology Taste Cell Development Regeneration Standardized Protocol Reproducible Higher Throughput Experiments Dissection Scissors Sterile DPBS Inter Malar Eminence Razor Blade Injection Enzyme Solution Circumvallate Papillae (CVP) Epithelium
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