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Journal / Biochemistry / Præcis og præcis enkeltmolekyle FRET-målinger ved …
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Biochemistry

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JoVE Journal Biochemistry
Præcis og præcis enkeltmolekyle FRET-målinger ved hjælp af Open Source smfBox
CD Spectroscopy to Study DNA-Protein Interactions
Journal (Biochemistry)
CD Spectroscopy to Study DNA-Protein Interactions
Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging
Journal (Biochemistry)
Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging
 
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Præcis og præcis enkeltmolekyle FRET-målinger ved hjælp af Open Source smfBox

Article DOI: 10.3791/62378-v • 07:12 min • July 5th, 2021
July 5th, 2021 •
*1, *1, 1, 1
1Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield
* These authors contributed equally

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Denne artikel indeholder trinvise instruktioner til at foretage fuldt korrigerede nøjagtige FRET-målinger på individuelle, frit diffuse biomolekyler ved hjælp af open source, billig smfBox, fra tænd, over justering og fokusering, til dataindsamling og -analyse.

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Biokemi enkeltmolekyle FRET mikroskopi fluorescens DNA biomolekylær kropsbygning konfokal strukturbestemmelse dynamik
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