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Establishment and Genetic Manipulation of Murine Hepatocyte Organoids
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Establishment and Genetic Manipulation of Murine Hepatocyte Organoids

Article DOI: 10.3791/62438-v • 14:54 min • February 12th, 2022
February 12th, 2022 •
*1,2, *1,3, 1,2, 1,2
1Key Laboratory of Experimental Teratology, Ministry of Education, Institute of Molecular Medicine and Genetics, School of Basic Medical Sciences, Stem Cell and Regenerative Medicine Research Center, Cheeloo College of Medicine, Shandong University, 2The Research Center of Stem Cell and Regenerative Medicine, Shandong University Cheeloo Medical College, School of Basic Medical Sciences, Shandong University
* These authors contributed equally

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Summary
Automatic Translation

A long-term ex vitro 3D organoid culture system was established from mouse hepatocytes. These organoids can be passaged and genetically manipulated by lentivirus infection of shRNA/ectopic construction, siRNA transfection and CRISPR-Cas9 engineering.

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Murine Hepatocyte Organoids Liver Metabolism Detoxification Regeneration Culture Hepatocytes In-vitro Genetic Manipulation Mature Hepatocytes Morphology Function Culture And Passage Genetic Modification Liver Perfusion Digestion Sterile Instruments Mouse Epidermal And Skin Opening Liver Pouch Oven Perfusion Speed Perfusion Buffer Soft Liver Cutting Liver Into Small Pieces Scissors Single Cell Formation Fitter Feeding Centrifugation
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