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JoVE Journal Bioengineering
Análisis constante de resorte molecular mediante espectroscopia de sonda de fuerza de biomembrana
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Análisis constante de resorte molecular mediante espectroscopia de sonda de fuerza de biomembrana

Article DOI: 10.3791/62490-v • 08:10 min • November 20th, 2021
November 20th, 2021 •
1, 1,2,3, 1, 1,4, 1, 5, 5, 1,2, 1,2,3
1School of Biomedical Engineering, Faculty of Engineering, The University of Sydney, 2Charles Perkins Centre, The University of Sydney, 3Heart Research Institute, 4Department of Chemistry, The Hong Kong University of Science and Technology, 5School of Aerospace, Mechanical and Mechatronic Engineering, Faculty of Engineering, The University of Sydney

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Una sonda de fuerza de biomembrana (BFP) es una técnica de espectroscopia de fuerza dinámica in situ (DFS). BFP se puede utilizar para medir la constante de resorte de las interacciones moleculares en las células vivas. Este protocolo presenta análisis constantes de resorte para enlaces moleculares detectados por BFP.

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Bioingeniería Número 177 Constante de resorte molecular Sonda de fuerza de biomembrana espectroscopia de fuerza dinámica ensayo de estiramiento integrina
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