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Biochemistry
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细菌中的RIBO-seq:NGS测序样本收集和库准备协议
 
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细菌中的RIBO-seq:NGS测序样本收集和库准备协议

Article DOI: 10.3791/62544-v 12:05 min August 7th, 2021
August 7th, 2021

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Summary

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在这里,我们描述了细菌中RIBO-seq样品收集和制备的阶段。根据这些准则对图书馆进行排序,可以获得足够的数据,以便进行全面的生物信息分析。我们目前的协议很简单,使用标准的实验室设备,从裂解到获得库需要七天时间。

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生物化学,第174期,
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