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JoVE Journal Environment
Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples
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Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples

Article DOI: 10.3791/62737-v • 04:32 min • October 14th, 2021
October 14th, 2021 •
1, 1, 1, 1, 2, 1
1Department of Microbiology, Universität Innsbruck, 2Department of Hygiene and Medical Microbiology, Medical University of Innsbruck

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Summary
Automatic Translation

A method for the extraction of cofactor F420 from pure cultures was optimized for the liquid chromatographic separation and analysis of F420 tail length in pure culture and environmental samples.

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Cofactor F420 Analysis Polyglutamate Tail Length Methanogenic Pure Cultures Environmental Samples Primary Metabolism Secondary Metabolism Prokaryotes Prevalence Function Sample Materials Sludge Soil Lysis Pre-purification Sample Preparation Buffers Conical Tube Lysis Buffer Distilled Water Concentration Vortex Autoclaving Forest Soil Volume Centrifuge Lysate Supernatant Solid-phase Extraction (SPE) Columns
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