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Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Article DOI: 10.3791/62836-v • 08:32 min • February 14th, 2022
February 14th, 2022 •
*1,2, *1, 1,3, 2,4, 1, 2,4, 1
1The Brady Urological Institute, Johns Hopkins University School of Medicine, 2Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), 3Department of Urology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 4Center for Soft and Living Matter, Institute for Basic Science (IBS)
* These authors contributed equally

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Summary
Automatic Translation

Extracellular vesicles (EVs) contribute to cellular biology and intercellular communications. There is a need for practical assays to visualize and quantify EVs uptake by the cells. The current protocol proposes the EV uptake assay by utilizing three-dimensional fluorescence imaging via confocal microscopy, following EV isolation by a nano-filtration-based microfluidic device.

Tags

Extracellular Vesicles EV Uptake Assay Confocal Microscope Imaging Analysis EV Labeling EV Tracking Analysis Volumetric Analysis Intercellular Communication Drug Delivery Cancer Biology Neuroscience Nanofiltration-based Microfluidic Device PBS Immunofluorescent Labeling
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