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Journal / Developmental Biology / In Situ Hybridization in Zebrafish Larvae and…
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Developmental Biology

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JoVE Journal Developmental Biology
In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development
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In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development

Article DOI: 10.3791/62930-v • 08:16 min • August 13th, 2021
August 13th, 2021 •
1, 1, 1, 1
1Department of Biology, Indiana University of Pennsylvania

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The zebrafish is an important model for understanding kidney development. Here, an in situ hybridization protocol is optimized to detect gene expression in zebrafish larvae and juveniles during mesonephros development.

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In Situ Hybridization Zebrafish Larvae Zebrafish Juveniles Mesonephros Development Gene Expression Metamorphosis Probe Penetration Kidney Visualization Adult Zebrafish Mating Petri Plates E3 Medium Larva Fixation Tricaine Immobilization Fixing Solution PBST Solution Larva Measurement
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