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JoVE Journal Neuroscience
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Magnetic Isolation of Microglial Cells from Neonate Mouse for Primary Cell Cultures

Article DOI: 10.3791/62964-v • 07:23 min • July 25th, 2022
July 25th, 2022 •
*1, *1,2, 1, 1, 1, 1, 1, 1, 1,2, 1, 1
1NeuroDiderot, Inserm UMR-1141, Hôpital Robert Debré 48, Université de Paris, 2Department of anesthesia and critical care, APHP-Sorbonne university
* These authors contributed equally

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Summary
Automatic Translation

Primary microglia cultures are commonly used to evaluate new anti-inflammatory molecules. The present protocol describes a reproducible and relevant method to magnetically isolate microglia from neonate pups.

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Magnetic Isolation Microglial Cells Neonate Mouse Primary Cell Cultures Culture Conditions In Vitro Characteristics Magnetic Cell Sorting Technology Serum-free Culture Medium Dissection Protocol Brain Dissection Dissociation Mixture Mechanical Dissociation Cell Transfer Cell Strainers HBSS With Calcium And Magnesium
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