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Evaluation of Amino Acid Consumption in Cultured Bone Cells and Isolated Bone Shafts
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Evaluation of Amino Acid Consumption in Cultured Bone Cells and Isolated Bone Shafts

Article DOI: 10.3791/62995-v • 06:32 min • April 13th, 2022
April 13th, 2022 •
1, 1,2
1Department of Internal Medicine, University of Texas Southwestern Medical Center, 2Charles and Jane Pak Center for Mineral Metabolism and Clinical Research, University of Texas Southwestern Medical Center

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Summary
Automatic Translation

This protocol presents a radiolabeled amino acid uptake assay, which is useful for evaluating amino acid consumption either in primary cells or in isolated bones.

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Amino Acid Consumption Cultured Bone Cells Isolated Bone Shafts Osteoblast Specification Differentiation Bone Formation Amino Acid Uptake Radiolabeled Amino Acids Amino Acid Uptake Assay Glucose Uptake Assay Fatty Acid Uptake Assay ST2 Cells Alpha MEM Medium FBS Penicillin Streptomycin Cell Culture Incubator Carbon Dioxide PBS Krebs-Ringer's HEPES (KRH) L34 Tritiated Glutamine
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