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Journal / Biochemistry / Provberedning med lipidmonalayermetod för…
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Biochemistry
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JoVE Journal Biochemistry
Provberedning med lipidmonalayermetod för elektronkristallkroografiska studier
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Provberedning med lipidmonalayermetod för elektronkristallkroografiska studier

Article DOI: 10.3791/63015-v • 04:22 min • November 20th, 2021
November 20th, 2021 •
1,2, 3, 1, 4, 1,2
1School of Molecular Sciences, Arizona State University, 2Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, 3Eyring Materials Center, Arizona State University, 4Department of Microbiology and Immunology, The Pennsylvania State University

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Lipidmonoskikt har använts som grund för att bilda tvådimensionella (2D) proteinkristaller för strukturella studier i årtionden. De är stabila vid luft-vatten-gränssnittet och kan fungera som ett tunt stödmaterial för elektronavbildning. Här presenterar vi de beprövade stegen för att förbereda lipidmonoskikt för biologiska studier.

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Biokemi nummer 177
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