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Journal / Neuroscience / Murine Retina'da AAV Transdüksiyon Verimliliğinin …
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Neuroscience
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JoVE Journal Neuroscience
Murine Retina'da AAV Transdüksiyon Verimliliğinin Nicelleştirilmesi için Dijital Damlacık PCR Yöntemi
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Murine Retina'da AAV Transdüksiyon Verimliliğinin Nicelleştirilmesi için Dijital Damlacık PCR Yöntemi

Article DOI: 10.3791/63038-v • 09:50 min • December 25th, 2021
December 25th, 2021 •
1,2, 1,2, 1,2, 1,2, 1,2
1Molecular Biology, Genetics and Bioengineering Program, Sabanci University, 2Nanotechnology Research and Application Center (SUNUM), Sabanci University

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Bu protokol, küçük ölçekli AAV üretimi, intravitreal enjeksiyon, retina görüntüleme ve retina genomik DNA izolasyonu ile birlikte dijital damlacık PCR (dd-PCR) kullanarak fare retinasında AAV transdüksiyon verimliliğinin nasıl ölçüleceğini sunar.

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