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Cancer Research

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JoVE Journal Cancer Research
Monitoring Breast Cancer Growth and Metastatic Colony Formation in Mice using Bioluminescence
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Monitoring Breast Cancer Growth and Metastatic Colony Formation in Mice using Bioluminescence

Article DOI: 10.3791/63060-v • 06:52 min • November 5th, 2021
November 5th, 2021 •
1, 1, 1, 1
1Department of Biochemistry and Molecular Biology, The Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School

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Summary
Automatic Translation

Here, we describe a noninvasive monitoring method involving luciferase and green fluorescent protein expression in various breast cancer cell lines. This protocol provides a technique to monitor tumor formation and metastatic colonization in real time in mice.

Tags

Breast Cancer Metastatic Colony Formation Bioluminescence Tumor Growth Kinetics Non-invasive Monitoring Luciferase And Fluorescence Detection Preclinical Studies Anticancer Drug Studies MCF-7 Cells MDA-MB-468 Cells MDA-MB-231 Cells GFP And Luciferase-positive Cells 96-well Plate DMEM Luciferin Solution PBS Bioluminescence Measurement
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