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Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
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Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Article DOI: 10.3791/63107-v • 08:23 min • February 16th, 2022
February 16th, 2022 •
1,3, 1,3, 2,3, 1,3
1Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, 2Department of Pediatrics, Massachusetts General Hospital, 3Harvard Medical School

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Summary
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Here, we present protocols for detecting nitric oxide and its biologically relevant derivatives using chemiluminescence-based assays with high sensitivity.

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Chemiluminescence-based Assays Detection Nitric Oxide Derivatives Autoxidation Nitrosated Compounds Chemiluminescence Detector Measurement Nitric Oxide Metabolites Sensitive Method Biological Sample Inhaled Nitric Oxide Therapeutic Gas Disease Progression Recovery Antimicrobial Airway Infectious Diseases DETA-NONOate Solution Sodium Hydroxide PBS Oxygen Line Intense-field Dielectric Filter Line
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