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Journal / Biochemistry / In Vitro Drug Screening Against All Life Cycle…
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Biochemistry

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JoVE Journal Biochemistry
In Vitro Drug Screening Against All Life Cycle Stages of Trypanosoma cruzi Using Parasites Expressing β-galactosidase
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An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
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In Vitro Drug Screening Against All Life Cycle Stages of Trypanosoma cruzi Using Parasites Expressing β-galactosidase

Article DOI: 10.3791/63210-v • 08:48 min • November 5th, 2021
November 5th, 2021 •
1,2, 1, 1, 3, 1,2, 1,2
1Área Parasitología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), 2Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, 3Instituto de Investigaciones Biomédicas (INBIOMED), Universidad de Buenos Aires (UBA) Facultad de Medicina, CONICET

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Summary
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We describe a high-throughput colorimetric assay measuring β-galactosidase activity in three life cycle stages of Trypanosoma cruzi, the causative agent of Chagas disease. This assay can be used to identify trypanocidal compounds in an easy, fast, and reproducible manner.

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In Vitro Drug Screening Trypanosoma Cruzi High Throughput Colorimetric Assay Chagas Disease Trypanocidal Compounds Romina Manarin Pamela Crib Beta-galactosidase Expressing Trypanosoma Cruzi Epimastigotes T-25 Tissue Culture Flask LIT Medium FCS Geneticin Sulfate Neubauer Chamber Log Phase Culture Suspension Of Epimastigote 96 Well Plate Vero Cell Suspension DMEM Supplemented With FCS
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