JoVE Journal
Medicine
Medicine
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
The protocol describes the isolation, culture, and profiling of endothelial cells from human mesenteric artery. Additionally, a method is provided to prepare human artery for spatial transcriptomics. Proteomics, transcriptomics, and functional assays can be performed on isolated cells. This protocol can be repurposed for any medium- or large-size artery.