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JoVE Journal Biochemistry
Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver
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Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver

Article DOI: 10.3791/63333-v • 12:07 min • February 18th, 2022
February 18th, 2022 •
1, 1, 1, 1, 1
1Research Institute for Biomedical Aging Research, University of Innsbruck

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Summary
Automatic Translation

This protocol describes how to extract fumarylacetoacetate hydrolase domain-containing protein 1 (FAHD1) from swine kidney and mouse liver. The listed methods may be adapted to other proteins of interest and modified for other tissues.

Tags

Extraction Purification FAHD1 Protein Swine Kidney Mouse Liver Recombinant Protein Bacteria Protein Lysis Buffer Sodium Fluoride Phenylmethylsulfonyl Fluoride Aprotinin Activated Orthovanadate Tissue Sample Ammonium Sulfate Solution
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