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Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein
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Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Article DOI: 10.3791/63405-v • 05:48 min • March 16th, 2022
March 16th, 2022 •
1, 1, 2, 1
1Nora Eccles Harrison Cardiovascular Research and Training Institute, University of Utah, 2Department of Biomedical Sciences, Cedars-Sinai Medical Center

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Summary
Automatic Translation

The present protocol describes a single M213L mutation in Gja1 that retains full-length Connexin43 generation but prevents translation of the smaller GJA1-20k internally translated isoform.

Tags

Internal Translational Start Site MRNA Full-length Protein Genotyping Single Residue Substitution Restriction Enzyme PCR-based Genotyping Mouse Model Point Of Mutation Tail Tip PCR Tube Tissue Lysis Solution Minicentrifuge Thermal Cycler PCR Solution Nuclease-free Water Forward And Reverse Primers PCR Master Mix
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