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JoVE Journal Neuroscience
Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis
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Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis

Article DOI: 10.3791/63483-v • 09:36 min • June 23rd, 2022
June 23rd, 2022 •
1, 1, 1, 1
1Center for Laser Microscopy, Institute for Physiology and Biochemistry “Jean Giaja”, University of Belgrade Faculty of Biology

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Summary
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We present here a protocol on how to prepare primary cultures of glial cells, astrocytes, and microglia from rat cortices for time-lapse video imaging of intracellular Ca2+ for research on pathophysiology of amyotrophic lateral sclerosis in the hSOD1G93A rat model.

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Primary Cultures Rat Astrocytes Microglia Amyotrophic Lateral Sclerosis Intercellular Calcium Signaling Personalized Medicine Neuroinflammatory Diseases Decapitating Pups Skull Exposure Petri Dish Forceps Meninges Removal Hippocampus Removal Cortex Homogenization
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