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JoVE Journal Biochemistry
Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
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Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

Article DOI: 10.3791/63495-v • 09:39 min • March 31st, 2022
March 31st, 2022 •
*1, *1, 2, 2, 1
1The Azrieli Faculty of Medicine in the Galilee, Bar-Ilan University, 2Asian Liver Center, Department of Surgery, Stanford University School of Medicine
* These authors contributed equally

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Summary
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Field-effect biosensing (FEB) is a label-free technique for detecting biomolecular interactions. It measures the electric current through the graphene biosensor to which the binding targets are immobilized. The FEB technology was used to evaluate biomolecular interactions between Hsp90 and Cdc37 and a strong interaction between the two proteins was detected.

Tags

Biomolecular Interaction Molecular Chaperone Hsp90 Client Protein Kinase Cdc37 Field-Effect Biosensing Technology FEB Label-free Cost-effective Interaction Detection Platform Validation Inhibitors Automated Software User-friendly Pipetting Platform Pharmaceuticals Biomolecular Analysis Small Molecules Peptides Proteins Drug Validation In Vitro Biological Activity In Vivo Efficacy Analyte Concentrations Experiment Optimization Reliable KD Value Chip Functionalization Process Buffer Exchange EDC Solution Sulfo-NHS Solution Biosensor Chip Activation
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