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Immunology and Infection
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JoVE Journal Immunology and Infection
Establishment of Deep Hypothermic Circulatory Arrest in Rats
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Establishment of Deep Hypothermic Circulatory Arrest in Rats

Article DOI: 10.3791/63571-v • 08:39 min • December 16th, 2022
December 16th, 2022 •
1, 1
1Department of Cardiopulmonary Bypass, National Center for Cardiovascular Disease and Fuwai Hospital, State Key Laboratory of Cardiovascular Medicine, Chinese Academy of Medical Science, Peking Union Medical College

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Summary
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This protocol presents the establishment of deep hypothermic circulatory arrest in rats, which can be applied to investigate systemic inflammatory response syndrome, ischemia/reperfusion injury, oxidative stress, neuroinflammation, etc.

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Deep Hypothermic Circulatory Arrest DHCA Rat Surgery Congenital Heart Disease Aortic Arch Disease Method Institutional Review Approval Institutional Animal Care And Use Committee Fuwai Hospital Chinese Academy Of Medical Sciences CPB Circuits Reservoir Roller Pump Heat Exchanger Membrane Oxygenator Connecting Tube Water Tank Hydroxy Starch Heparin Sodium Saline Priming Solution Anesthesia Cannulation Ventilator Parameters Electric Heating Blanket Ophthalmic Ointment Shave Hair Disinfect Skin Aldehyde And Alcohol
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