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In vitro Apical-Out Enteroid Modell av nekrotiserende enterokolitt
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In vitro Apical-Out Enteroid Modell av nekrotiserende enterokolitt

Article DOI: 10.3791/64003-v • 09:11 min • June 8th, 2022
June 8th, 2022 •
1, 1, 1
1Department of Pediatrics, Division of Neonatal-Perinatal Medicine, University of Oklahoma Health Sciences Center and Center for Pregnancy and Newborn Health

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Denne protokollen beskriver en apikal-ut nekrotiserende enterokolitt (NEC) -i-en-parabol-modell som bruker små intestinale enteroider med reversert polaritet, noe som gir tilgang til den apikale overflaten. Vi tilbyr en immunfluorescerende fargeprotokoll for å oppdage NEC-relatert epitelforstyrrelse og en metode for å bestemme levedyktigheten til apikale enteroider utsatt for NEC-in-a-dish-protokollen.

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