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Developmental Biology

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JoVE Journal Developmental Biology

소 난모세포에서 미토콘드리아 DNA를 감소시키기 위한 Bisection의 사용

Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders

Journal (Developmental Biology)

Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders

FACS Enrichment of Zebrafish Gut Cell Suspension for scRNAseq (Video) | JoVE

Journal (Developmental Biology)

FACS Enrichment of Zebrafish Gut Cell Suspension for scRNAseq (Video) | JoVE

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소 난모세포에서 미토콘드리아 DNA를 감소시키기 위한 Bisection의 사용

Article DOI: 10.3791/64060-v • 06:15 min • July 6th, 2022

July 6th, 2022 •

Laura Adams*¹, Ying Liu*¹, Barbara Durrant², Elena Ruggeri², Carly Young², Rebecca Krisher³, Irina Polejaeva¹

¹Department of Animal, Dairy, and Veterinary Sciences, Utah State University, ²Reproductive Sciences, Beckman Center for Conservation Research, San Diego Zoo Wildlife Alliance, ³Genus PLC

* These authors contributed equally

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여기에서, 소 난모세포에서 미토콘드리아 DNA 카피 수를 유의하게 감소시키기 위한 프로토콜을 제시한다(P< 0.0001). 이 방법은 원심분리 및 이절제를 사용하여 난모세포 미토콘드리아를 실질적으로 감소시키고 재구성 된 종간 체세포 핵 전달 배아에서 발달 가능성을 높일 수 있습니다.

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