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JoVE Journal Neuroscience
Colture organotipiche di espianti retinici da scimmia macaco
Purification of Fibroblasts and Schwann Cells from Sensory and Motor Nerves in Vitro
Journal (Neuroscience)
Purification of Fibroblasts and Schwann Cells from Sensory and Motor Nerves in Vitro
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Journal (Neuroscience)
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Colture organotipiche di espianti retinici da scimmia macaco

Article DOI: 10.3791/64178-v • 10:32 min • August 24th, 2022
August 24th, 2022 •
1, 1, 1, 1, 2, 1
1Yunnan Eye Institute & Key Laboratory of Yunnan Province, Yunnan Eye Disease Clinical Medical Center, Affiliated Hospital of Yunnan University, Yunnan University, 2Institute for Ophthalmic Research, Eberhard-Karls-Universität Tübingen

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Gli espianti retinici ottenuti da macachi selvatici sono stati coltivati in vitro. La degenerazione retinica e la via di segnalazione cGMP-PKG sono state indotte utilizzando l'inibitore della PDE6 zaprinast. L'accumulo di cGMP negli espianti a diverse concentrazioni di zaprinast è stato verificato utilizzando l'immunofluorescenza.

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Neuroscienze Numero 186
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